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Stationary stage chemistry dictates the affinity of the sample parts to stick or keep on the column as being the mobile period moves the sample in the column. Due to this fact, the sample components traverse the column and elute at different rates.The obvious way to value the theoretical and the practical information reviewed With this segment is a
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Mikhail Semyonovich Tsvet receives credit for inventing liquid column chromatography. In 1901, he introduced an adsorption chromatography approach for separating plant pigments with petroleum ether inside a slim glass tube filled with calcium carbonate.Solute ions billed the same as the ions within the column are repulsed and elute with out retenti
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